One of the success factors of an innovation ecosystem is the willingness of its actors to cooperate, which depends on a number of factors. As usual, cooperation needs a common language among the concerned parties and opposite sides, the identification of serious differences of actors might contribute to preparingand managinga collaborative culture. In the present analysis, the authors approach the topic from an operational management perspective and examine the actors of an ecosystem through the elements of a general service management framework model. Based on this, it is possible to point out differences through various factors to determine possible conflict sources. The aim of the study is to show a method for the identification ofpotential points of inconsistency along the four groups of aspects as potential sources of barriers to collaboration. After literature review, the value system, the operational-business phi-losophy, the methods and the objectives of a research and technology center are analyzed. The presented approach can serve as a general method for identifying inconsistencies in innovation ecosystems. The current methodology is based on a two-dimension one-by-one analysis, further research can extend the approach to a multi-player inconsistency evaluation tool.